Imagine you’ve just titrated 50 compounds using any modern assay and instrumentation. The software is certainly capable of automatically extracting the response for each concentration and fitting it to extract the affinity/potency. You might then see the curve above (Figure 1) and the fit values in a table. If the affinity or potency is in the range you expect, with a quick look, the binding curve might pass muster with a possible note that the compound has some solubility issues at the highest concentration used. You might then pass on the data to the medicinal chemist, unfortunately that would be a mistake…