Can Surface Plasmon Resonance provide a biologically relevant assay readout?

Is your biochemical assay not sensitive enough to characterize inhibitors with affinities of hundreds of µM?  Does your assay suffer from false readout from the intrinsic fluorescence of compounds at high concentration?  Did you know that Surface Plasmon Resonance (SPR) can be set up in such a way that the readout represents the biologically relevant interaction of two biological partners, and therefore it can directly detect compounds disrupting such interactions?

Know your data

Imagine you’ve just titrated 50 compounds using any modern assay and instrumentation. The software is certainly capable of automatically extracting the response for each concentration and fitting it to extract the affinity/potency. You might then see the curve above (Figure 1.) and the fit values in a table. If the affinity or potency is in the range you expect, with a quick look, the binding curve…